Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts.
Identifieur interne : 000119 ( Main/Exploration ); précédent : 000118; suivant : 000120Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts.
Auteurs : Hanna Korajoki [Finlande] ; Matti VornanenSource :
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology [ 1432-136X ] ; 2013.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Analyse de séquence d'ADN (MeSH), Animaux (MeSH), Carpes (poisson) (métabolisme), Données de séquences moléculaires (MeSH), Femelle (MeSH), Myocarde (enzymologie), Mâle (MeSH), Salmonidae (métabolisme), Sarcoplasmic Reticulum Calcium-Transporting ATPases (génétique), Sarcoplasmic Reticulum Calcium-Transporting ATPases (métabolisme), Séquence nucléotidique (MeSH), Température (MeSH).
- MESH :
- enzymologie : Myocarde.
- génétique : Sarcoplasmic Reticulum Calcium-Transporting ATPases.
- métabolisme : ARN messager, Carpes (poisson), Salmonidae, Sarcoplasmic Reticulum Calcium-Transporting ATPases.
- Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Femelle, Mâle, Séquence nucléotidique, Température.
English descriptors
- KwdEn :
- Animals (MeSH), Base Sequence (MeSH), Carps (metabolism), Female (MeSH), Male (MeSH), Molecular Sequence Data (MeSH), Myocardium (enzymology), RNA, Messenger (metabolism), Salmonidae (metabolism), Sarcoplasmic Reticulum Calcium-Transporting ATPases (genetics), Sarcoplasmic Reticulum Calcium-Transporting ATPases (metabolism), Sequence Analysis, DNA (MeSH), Temperature (MeSH).
- MESH :
- chemical , genetics : Sarcoplasmic Reticulum Calcium-Transporting ATPases.
- chemical , metabolism : RNA, Messenger, Sarcoplasmic Reticulum Calcium-Transporting ATPases.
- enzymology : Myocardium.
- metabolism : Carps, Salmonidae.
- Animals, Base Sequence, Female, Male, Molecular Sequence Data, Sequence Analysis, DNA, Temperature.
Abstract
Cardiac function in fish acclimates to long-term temperature shifts by generating compensatory changes in structure and function of sarcoplasmic reticulum (SR) including the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA2). The current study compares temperature responses of the cardiac SERCA in two fish species, burbot (Lota lota) and crucian carp (Carassius carassius), which differ in regard to thermal tolerance and activity pattern. Burbot are cold stenothermal and cold-active, while crucian carp are eurythermal and cold-dormant. The fish were acclimated at 4 °C (cold-acclimation, CA) or 18 °C (warm-acclimation, WA) and expression of SERCA proteins and transcript was measured from atrium and ventricle. Burbot heart expresses one major isoform of SERCA (110 kDa), while crucian carp heart expresses two isoforms (110 and 93 kDa). Expression of SERCA proteins was about four times higher (P < 0.05) in the heart of CA burbot than WA burbot, in both cardiac chambers. In the heart of crucian carp, thermal acclimation did not affect SERCA proteins, in either chamber (P > 0.05). The expression of SERCA transcripts did not follow the expression pattern of SERCA protein in either species, suggesting that SERCA expression is mainly regulated posttranscriptionally. These findings show that the stenothermal and cold-active burbot compensates for the decrease in ambient temperature by increasing the expression of SERCA. In the eurythermal and cold-dormant crucian carp SERCA expression is independent of temperature, while the presence of two SERCA isoforms may provide some thermal independence in SR Ca(2+) pumping.
DOI: 10.1007/s00360-012-0724-1
PubMed: 23239195
Affiliations:
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Box 111, 80101 Joensuu</wicri:regionArea><wicri:noRegion>80101 Joensuu</wicri:noRegion></affiliation></author><author><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="doi">10.1007/s00360-012-0724-1</idno><idno type="RBID">pubmed:23239195</idno><idno type="pmid">23239195</idno><idno type="wicri:Area/PubMed/Corpus">000037</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000037</idno><idno type="wicri:Area/PubMed/Curation">000037</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000037</idno><idno type="wicri:Area/PubMed/Checkpoint">000028</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000028</idno><idno type="wicri:Area/Ncbi/Merge">000269</idno><idno type="wicri:Area/Ncbi/Curation">000269</idno><idno type="wicri:Area/Ncbi/Checkpoint">000269</idno><idno type="wicri:Area/Main/Merge">000119</idno><idno type="wicri:Area/Main/Curation">000119</idno><idno type="wicri:Area/Main/Exploration">000119</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts.</title><author><name sortKey="Korajoki, Hanna" sort="Korajoki, Hanna" uniqKey="Korajoki H" first="Hanna" last="Korajoki">Hanna Korajoki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, University of Eastern Finland, P.O. Box 111, 80101 Joensuu, Finland. hanna.korajoki@uef.fi</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Biology, University of Eastern Finland, P.O. Box 111, 80101 Joensuu</wicri:regionArea><wicri:noRegion>80101 Joensuu</wicri:noRegion></affiliation></author><author><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name></author></analytic><series><title level="j">Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology</title><idno type="eISSN">1432-136X</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Base Sequence (MeSH)</term><term>Carps (metabolism)</term><term>Female (MeSH)</term><term>Male (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Myocardium (enzymology)</term><term>RNA, Messenger (metabolism)</term><term>Salmonidae (metabolism)</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases (genetics)</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases (metabolism)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Animaux (MeSH)</term><term>Carpes (poisson) (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Femelle (MeSH)</term><term>Myocarde (enzymologie)</term><term>Mâle (MeSH)</term><term>Salmonidae (métabolisme)</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases (génétique)</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases (métabolisme)</term><term>Séquence nucléotidique (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Myocarde</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Myocardium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Carps</term><term>Salmonidae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Carpes (poisson)</term><term>Salmonidae</term><term>Sarcoplasmic Reticulum Calcium-Transporting ATPases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Female</term><term>Male</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Mâle</term><term>Séquence nucléotidique</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cardiac function in fish acclimates to long-term temperature shifts by generating compensatory changes in structure and function of sarcoplasmic reticulum (SR) including the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA2). The current study compares temperature responses of the cardiac SERCA in two fish species, burbot (Lota lota) and crucian carp (Carassius carassius), which differ in regard to thermal tolerance and activity pattern. Burbot are cold stenothermal and cold-active, while crucian carp are eurythermal and cold-dormant. The fish were acclimated at 4 °C (cold-acclimation, CA) or 18 °C (warm-acclimation, WA) and expression of SERCA proteins and transcript was measured from atrium and ventricle. Burbot heart expresses one major isoform of SERCA (110 kDa), while crucian carp heart expresses two isoforms (110 and 93 kDa). Expression of SERCA proteins was about four times higher (P < 0.05) in the heart of CA burbot than WA burbot, in both cardiac chambers. In the heart of crucian carp, thermal acclimation did not affect SERCA proteins, in either chamber (P > 0.05). The expression of SERCA transcripts did not follow the expression pattern of SERCA protein in either species, suggesting that SERCA expression is mainly regulated posttranscriptionally. These findings show that the stenothermal and cold-active burbot compensates for the decrease in ambient temperature by increasing the expression of SERCA. In the eurythermal and cold-dormant crucian carp SERCA expression is independent of temperature, while the presence of two SERCA isoforms may provide some thermal independence in SR Ca(2+) pumping.</div></front></TEI><affiliations><list><country><li>Finlande</li></country></list><tree><noCountry><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name></noCountry><country name="Finlande"><noRegion><name sortKey="Korajoki, Hanna" sort="Korajoki, Hanna" uniqKey="Korajoki H" first="Hanna" last="Korajoki">Hanna Korajoki</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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